In Vitro Screening of Seed Extracts of Medicinal Plants for Protease Inhibitory Activity

  • Faiyaz Khudaboddin Shaikh (1) Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India; (2) Department of Biotechnology, MGM’s Institute of Biosciences and Technology, Aurangabad, Maharashtra, India
  • Sarwan W. Bradosty Department of Biology, Cihan University-Erbil, Kurdistan Region, Iraq
  • Saber W. Hamad Department of Field Crops Production, College of Agriculture, Salahaddin University - Erbil, Erbil, Kurdistan Region, Iraq
  • Ashok A. Shinde Department of Biotechnology, MGM’s Institute of Biosciences and Technology
Keywords: Dot blot, protease inhibitors, Proteases, Protein, Trypsin


Protease inhibitors (PIs) are deployed in the plant kingdom as storage proteins or peptides, regulators of endogenous proteases, and plant protection agents against insect pests and pathogen attack. In humans, they are identified as chemopreventive agents against a range of cancers and have potential as drug to treat an array of disease associated with aberrant activity of proteases. The present investigation reports PIs activity data from 30 medicinal plants. The screening for PIs activity was done by dot blot assay using X-ray film coated with gelatin. Among screened seed extracts, Albizia lebbeck, Raphanus sativus, Mucuna pruriens, Achyranthes aspera, and Coffea arabica showed high inhibitory activities with trypsin protease. Most of seed extracts exhibited moderate activity, whereas Ocimum sanctum showed moderate to low activity against trypsin. The presence of varied protein content is reported from all seed extracts with highest in A. lebbeck (50.0 ± 3.4 mg/ml). The data produced in the present investigation could be helpful for further exploration of PIs as therapeutic agent.


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How to Cite
Shaikh F, Bradosty S, Hamad S, Shinde A. In Vitro Screening of Seed Extracts of Medicinal Plants for Protease Inhibitory Activity. cuesj [Internet]. 30Jun.2019 [cited 28Jan.2022];3(1):61-5. Available from:
Research Article