In Vitro Screening of Seed Extracts of Medicinal Plants for Protease Inhibitory Activity

Authors

  • Faiyaz Khudaboddin Shaikh (1) Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India; (2) Department of Biotechnology, MGM’s Institute of Biosciences and Technology, Aurangabad, Maharashtra, India
  • Sarwan W. Bradosty Department of Biology, Cihan University-Erbil, Kurdistan Region, Iraq https://orcid.org/0000-0002-4659-4081
  • Saber W. Hamad Department of Field Crops Production, College of Agriculture, Salahaddin University - Erbil, Erbil, Kurdistan Region, Iraq
  • Ashok A. Shinde Department of Biotechnology, MGM’s Institute of Biosciences and Technology

DOI:

https://doi.org/10.24086/cuesj.v3n1y2019.pp61-65

Keywords:

Dot blot, protease inhibitors, Proteases, Protein, Trypsin

Abstract

Protease inhibitors (PIs) are deployed in the plant kingdom as storage proteins or peptides, regulators of endogenous proteases, and plant protection agents against insect pests and pathogen attack. In humans, they are identified as chemopreventive agents against a range of cancers and have potential as drug to treat an array of disease associated with aberrant activity of proteases. The present investigation reports PIs activity data from 30 medicinal plants. The screening for PIs activity was done by dot blot assay using X-ray film coated with gelatin. Among screened seed extracts, Albizia lebbeck, Raphanus sativus, Mucuna pruriens, Achyranthes aspera, and Coffea arabica showed high inhibitory activities with trypsin protease. Most of seed extracts exhibited moderate activity, whereas Ocimum sanctum showed moderate to low activity against trypsin. The presence of varied protein content is reported from all seed extracts with highest in A. lebbeck (50.0 ± 3.4 mg/ml). The data produced in the present investigation could be helpful for further exploration of PIs as therapeutic agent.

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Published

2019-06-30

How to Cite

1.
Shaikh FK, Bradosty SW, Hamad SW, Shinde AA. In Vitro Screening of Seed Extracts of Medicinal Plants for Protease Inhibitory Activity. Cihan U Erbil SCI J [Internet]. 2019 Jun. 30 [cited 2026 Jun. 23];3(1):61-5. Available from: https://journals.cihanuniversity.edu.iq/index.php/cuesj/article/view/93

Issue

Vol. 3 No. 1 (2019)

Section

Research Article

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