Inhibition of Rac 1 Protect Against Platelet Induced Liver and Kidney ‎Injury in Diabetes Mellitus

Keywords: Rac1‎, Platelet, CXCL4‎, Diabetes Mellitus, Liver and Kidney Injury ‎


Diabetes mellitus both (Type 1 and Type 2) are one of the common causes for activation of platelet. Inflammation-induced abnormal platelet function contributes to chronic complications, which are the leading causes of death and morbidity among diabetics. Rac1 has been shown to regulate a variety of platelet functions; predicted Rac1could regulate platelet release of CXCL4, which leads to kidney injury in Diabetes Mellitus. Diabetes mellitus' effect on Rac1 activation, a 21kD G-protein implicated in platelet activation, was investigated and platelet induced inflammation and kidney injury. Swiss albino male mice were pretreated with 5 mg/kg of a specific Rac1 inhibitor NSC23766 and injected with (45 mg/kg body wt.) streptozotocin, twice for five days. Moreover, the concentration of serum chemokines CXCL4 were assayed using ELISA and histology score for kidney were examined. Our results showed that Diabetes mellitus was induced in mice by streptozotocin. In addition, platelet chemokines (CXCL4) were markedly higher in diabetic mice when compared to the sham (control) group. Moreover, pretreatment with NSC23766 decreased liver and kidney injury assessed by histology score, P-value <0.05. Our study reveals that Rac1 has a critical role in platelet chemokines secretion due to diabetes-induced inflammation in the liver and kidneys, targeting Rac1 could be a target for innovative treatment to control inflammation in diabetic individual. Targeting platelets involved in inflammatory pathways could be part of a strategy in order to control and manage diabetes and its consequences.


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Author Biographies

Rundk A. Hwaiz, Department of Clinical Biochemistry, Hawler Medical University, Kurdistan Region, Iraq

Dr. Rundk Ahmad Hwaiz is Assistant professor in Medical Biochemistry at Hawler Medical University. She is Head of Nutrition and Dietetics Department in College of Health Sciences- Hawler Medical University. In 2015 she finished her PhD degree in Medical Sciences at Surgery Section-College of Medicine-Lund University-Sweden. She did post doctorate at Malmo University in 2017-2019 in prostate and skin cancer. Her research interest is sepsis, pancreatitis, ketogenic diet, metabolic syndrome, COVID-19, and cancer. She has collaborated actively with researchers in several other disciplines of biomedicine on finding molecular mechanisms of the metabolic and inflammatory disease.

Helen J. Sabri, Department of Medical Biochemical Analysis, Cihan University-Erbil, Kurdistan Region, Iraq

Helen Jawdat Sabri is an assistant lecturer (Full) in Medical Biochemical Analysis at Cihan University. In 2022 she finished MSc in clinical Biochemistry at Hawler Medical University, College of health sciences. Her research interest is Clinical Biochemistry, Diabetes, and cancer.


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How to Cite
Hwaiz R, Sabri H. Inhibition of Rac 1 Protect Against Platelet Induced Liver and Kidney ‎Injury in Diabetes Mellitus. cuesj [Internet]. 1Mar.2023 [cited 30Mar.2023];7(1):29-4. Available from:
Research Article