Radish Juice Promote Kidney Stone Deposition in Ethylene Glycol-induced Urolithiasis in Rats

  • Falah M. Aziz Department of Biology, College of Science, Salahaddin University-Erbil, Erbil, Iraq
  • Dlshad H. Hassan Department of Biology, Faculty of Science, Soran University, Soran, Iraq
Keywords: Ethylene glycol, Kidneystone, Radis, Urolithiasis

Abstract

Urolithiasis is a well-known problem that stones could form in various parts of the urinary system and it is the most common disease of the urinary tract. The current study was planned to investigate the effect of radish juice on ethylene glycol (EG)-induced urolithiasis. Twenty-one rats randomly divided into three groups. The first group was the control group was received normal standard diet and drinking water and the second group represented the model group received 0.75% EG in drinking water ad libitum. The third group received radish juice (2 ml/kg of body weight) by gavage plus EG (0.75%) in drinking water ad libitum. The experiment was conducted for 28 days. The light microscope examination revealed a disturbed histological architecture of the kidney tissues, including dilated renal tubules, aggregation of infiltrated leukocytes inflammatory cells, and crystal deposition in the model group. The EG plus radish juice treated rats showed higher crystal density with improved renal tubule structure and alleviated inflammation. Both treated groups showed various biochemical alterations compared to control group, but the most interest biochemical result was the significant decrease of malondialdehyde, a lipid peroxidation marker, and in the radish plus EG group compared to the EG group. Scanning electron microscopy showed clear structural detail about calcium oxalate crystals in which radish-treated group showed higher crystal deposition and calcified tissue compared to EG group. The present study concluded that radish juice promotes stone deposition but exerted an antioxidant effect.

Downloads

Download data is not yet available.

References

K. Kaczmarek, A. Gołąb, M. Soczawa and M. Słojewski. “Urethral stone of unexpected size: Case report and short literature review”. Open Medicine, vol. 11, pp. 7-10, 2016.

M. López and B. Hoppe. “History, epidemiology and regional diversities of urolithiasis”. Pediatric Nephrology, vol. 25, p. 49, 2010.

C. Chauhan, M. Joshi and A. Vaidya. “Growth inhibition of struvite crystals in the presence of herbal extract Commiphora wightii”. Journal of Materials Science: Materials in Medicine, vol. 20, p. 85, 2009.

K. G. Ingale, P. A. Thakurdesai and N. S. Vyawahare. “Effect of Hygrophila spinosa in ethylene glycol induced nephrolithiasis in rats”. Indian Journal of Pharmacology, vol. 44, p. 639, 2012.

D. R. Basavaraj, C. S. Biyani, A. J. Browning and J. J. Cartledge. “The role of urinary kidney stone inhibitors and promoters in the pathogenesis of calcium containing renal stones”. EAU-EBU Update Series, vol. 5, pp. 126-136, 2007.

F. L. Coe, A. Evan and E. Worcester. “Kidney stone disease”. The Journal of Clinical Investigation, vol. 115, pp. 2598-2608, 2005.

K. P. Aggarwal, S. Narula, M. Kakkar and C. Tandon. “Nephrolithiasis: Molecular mechanism of renal stone formation and the critical role played by modulators”. BioMed Research International, vol. 2013, p. 292953, 2013.

M. S. Parmar. “Kidney stones”. BMJ: British Medical Journal, vol. 328, p. 1420, 2004.

P. Das, K. Kumar, A. Nambiraj, R. Awasthi, K. Dua and H. Malipeddi. “Antibacterial and in vitro growth inhibition study of struvite urinary stones using Oxalis corniculata Linn. leaf extract and its biofabricated silver nanoparticles”. Recent Patents on Drug Delivery and Formulation, vol. 23, pp. 170-178, 2018.

N. D. Pucci, G. S. Marchini, E. Mazzucchi, S. T. Reis, M. Srougi, D. Evazian and W. C. Nahas. “Effect of Phyllanthus niruri on metabolic parameters of patients with kidney stone: A perspective for disease prevention”. International Brazilian Journal of Urology, vol. 44, pp. 758-764, 2018.

I. Sharma, W. Khan, R. Parveen, M. J. Alam, I. Ahmad, M. H. Ansari and S. Ahmad. “Antiurolithiasis activity of bioactivity guided fraction of Bergenia ligulata against ethylene glycol induced renal calculi in rat”. BioMed Research International, vol. 2017, p. 1-11, 2017.

P. Hayatdavoudi, A. K. Rad, Z. Rajaei and M. A. R. Hadjzadeh. “Renal injury, nephrolithiasis and Nigella sativa: A mini review”. Avicenna Journal of Phytomedicine, vol. 6, p. 1, 2016.

J. Glimn-Lacy and P. B. Kaufman. “Botany Illustrated: Introduction to Plants, Major Groups, Flowering Plant Families”. New York: Springer Science and Business Media, 2006.

D. Zohary, M. Hopf and E. Weiss. “Domestication of Plants in the Old World: The Origin and Spread of Domesticated Plants in Southwest Asia, Europe, and the Mediterranean Basin”. Oxford: University Press on Demand, 2012.

S. Alqasoumi, M. Al-Yahya, T. Al-Howiriny and S. Rafatullah. “Gastroprotective effect of radish Raphanus sativus L. on experimental gastric ulcer models in rats”. Farmacia, vol. 56, p. 204, 2008.

S. A. Banihani. “Radish (Raphanus sativus) and diabetes”. Nutrients, vol. 9, p. 1014, 2017.

S. S. Beevi, L. N. Mangamoori, V. Dhand and D. S. Ramakrishna. “Isothiocyanate profile and selective antibacterial activity of root, stem, and leaf extracts derived from Raphanus sativus L”. Foodborne Pathogens and Disease, vol. 6, pp. 129-136, 2009.

L. M. Besenhofer, M. C. Cain, C. Dunning and K. E. McMartin. “Aluminum citrate prevents renal injury from calcium oxalate crystal deposition”. Journal of the American Society of Nephrology, vol. 23, pp. 2024-2033, 2012.

J. Brent. “Current management of ethylene glycol poisoning”. Drugs, vol. 61, pp. 979-988, 2001.

H. Han, A. M. Segal, J. L. Seifter and J. T. Dwyer. “Nutritional management of kidney stones (nephrolithiasis)”. Clinical Nutrition Research, vol. 4, pp. 137-152, 2015.

J. Ran, J. Ma, Y. Liu, R. Tan, H. Liu and G. Lao. “Low protein diet inhibits uric acid synthesis and attenuates renal damage in streptozotocin-induced diabetic rats”. Journal of Diabetes Research, vol. 2014, pp. 1-10, 2014.

G. Shyamala and P. Singh. “An analysis of chemical constituents of Raphanus sativus”. Proceedings of the National Academy of Sciences, India Section B, vol. 57, pp. 157-159, 1987.

P. Ashok, B. C. Koti and A. Vishwanathswamy. “Antiurolithiatic and antioxidant activity of Mimusops elengi on ethylene glycolinduced urolithiasis in rats”. Indian Journal of Pharmacology, vol. 42, p. 380, 2010.

F. A. Ghaeni, B. Amin, A. T. Hariri, N. T. Meybodi and H. Hosseinzadeh. “Antilithiatic effects of crocin on ethylene glycol-induced lithiasis in rats”. Urolithiasis, vol. 42, pp. 549-558, 2014.

M. Gandhi, M. Aggarwal, S. Puri and S. Singla. “Prophylactic effect of coconut water (Cocos nucifera L.) on ethylene glycol induced nephrocalcinosis in male wistar rat”. International Braz J Urol, vol. 39, pp. 108-117, 2013.

H. J. Lee, S. J. Jeong, M. N. Park, M. Linnes, H. J. Han, J. H. Kim, J. C. Lieske and S. H. Kim. “Gallotannin suppresses calcium oxalate crystal binding and oxalate-induced oxidative stress in renal epithelial cells”. Biological and Pharmaceutical Bulletin, vol. 35, pp. 539-544, 2012.

R. Goyeneche, S. Roura, A. Ponce, A. Vega-Gálvez, I. Quispe-Fuentes, E. Uribe and K. Di Scala. “Chemical characterization and antioxidant capacity of red radish (Raphanus sativus L.) leaves and roots”. Journal of Functional Foods, vol. 16, pp. 256-264, 2015.

D. H. Hassan and F. M. Aziz. “Histological and ultrastructural study on the effect of celery juice on ethylene glycol induced urolithiasis in male albino rats”. ZANCO Journal of Pure and Applied Sciences, vol. 29, pp. 1-12, 2017.

S. R. Mulay, O. P. Kulkarni, K. V. Rupanagudi, A. Migliorini, M. N. Darisipudi, A. Vilaysane, D. Muruve, Y. Shi, F. Munro and H. Liapis. “Calcium oxalate crystals induce renal inflammation by NLRP3-mediated IL-1β secretion”. The Journal of Clinical Investigation, vol. 123, pp. 236-246, 2012.

M. L. Green, R. W. Freel and M. Hatch. “Lipid peroxidation is not the underlying cause of renal injury in hyperoxaluric rats”. Kidney International, vol. 68, pp. 2629-2638, 2005.

R. M. P. Gutiérrez and R. L. Perez. “Raphanus sativus (Radish) Their chemistry and biology”. The Scientific World Journal,

vol. 4, pp. 811-837, 2004.

Published
2020-01-17
How to Cite
Aziz, F., & Hassan, D. (2020). Radish Juice Promote Kidney Stone Deposition in Ethylene Glycol-induced Urolithiasis in Rats. Cihan University-Erbil Scientific Journal, 4(1), 57-61. https://doi.org/10.24086/cuesj.v4n1y2020.pp57-61
Section
Research Article