Different Concentrations of Honey's Antimicrobial Activity Against ‎Staphylococcus Aureus by HPLC Determination

  • Raid D. Thanoon Department of Medical Biochemical Analysis, Cihan University-Erbil, Kurdistan Region, Iraq https://orcid.org/0000-0001-7584-963X
  • Abdalmuhaymen S. Alshaeen ‎Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq
  • Hasnaa ‎ R. Abdullah Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq
  • Laith A. Abdulhussain Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq
  • Wurood ‎ J. Mohammed Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq
  • Abdullah‎ B. Hassan Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq
Keywords: Honey, Staphylococcus aureus ‎, Antimicrobial activity ‎, Antibiotics, high-performance liquid chromatography test

Abstract

Honey has been used in ancient times as a treatment. It is used for healing wounds and also can be used as antiseptic to kill bacteria and other microorganisms. The aim of this study was to examine the activity of honey as a bactericidal and bacteriostatic and to measure the most active compounds of honey with highly bacterial inhibition zone using HPLC technique. In this experiment and the honey was used to check whether the honey can be used as an antimicrobial agent or not. First by well diffusion method is done by adding different concentrations of honey (25, 75, 100, 125, 150, 200, microliter). Th results showed a little inhibition zone for the three types of honey but in different size for each type. The industrial honey showed the largest effect. Different concentrations were used (10 %, 30 % 50%). The antibiotic sensitivity was applied, the result showed only two resistant antibiotics (Azithromycin and Erythromycin). The concentration of Catalase, Amylase, Invertase, Glucose oxidase in honey tested by HPLC and showed the concentration of each substance cons of catalase = (32877 ÷ 170253 ) x 20 = 3.862 u/ml; cons of amylase = (136985 ÷ 180849) x 20 = 15.149 u/ml; cons of Invertase = (58466  ÷ 193624) x 20 = 6.039 u/ml; cons of glucose peroxide = (105204 ÷ 163245) x 20 =12.889 u/ml. Moreover, the presence of gluconic acid ‘Organic acid’ gives the honey its acidic characteristic which is about 3.2 pH.

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Author Biographies

Raid D. Thanoon, Department of Medical Biochemical Analysis, Cihan University-Erbil, Kurdistan Region, Iraq

Raid D. Thanoon is a Lecturer at the Department of Medical Biochemical Analysis, College of Health technology, Cihan University-Erbil. He got the B.B.E. Biology degree in Mosul University 2009, the M.Sc. degree in Microbiology 2012 and the Ph.D. degree in Biotechnology 2018. His research interests are in Microbiology, Biotechnology and Molecular Biology

Abdalmuhaymen S. Alshaeen, ‎Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq

Abdalmuhaymen S. Alshaeen is an under graduate student at the Department of General Biology, College of Science, Cihan University-Erbil. He got the BSc. In Biology degree in Cihan University-Erbil 2021. His research interests are in Microbiology and immunology.

Hasnaa ‎ R. Abdullah, Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq

Hasnaa R. Abdullah is an under graduate student at the Department of General Biology, College of Science, Cihan University-Erbil. She got the BSc. In Biology degree in Cihan University-Erbil 2021. Her research interests are in Microbiology and hematology.

Laith A. Abdulhussain, Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq

Laith A. Abdulhussain is an under graduate student at the Department of General Biology, College of Science, Cihan University-Erbil. He got the BSc. In Biology degree in Cihan University-Erbil 2021. His research interests are in Microbiology and Genetics.

Wurood ‎ J. Mohammed, Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq

Wurood J. Mohammed is an under graduate student at the Department of General Biology, College of Science, Cihan University-Erbil. She got the BSc. In Biology degree in Cihan University-Erbil 2021. Her research interests are in Microbiology and Immunology.

 

Abdullah‎ B. Hassan, Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq

Abdullah B. Hassan is an under graduate student at the Department of General Biology, College of Science, Cihan University-Erbil. He got the BSc. In Biology degree in Cihan University-Erbil 2021. His research interests are in Microbiology and Biotehnology.

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Published
2022-09-25
How to Cite
1.
Thanoon R, Alshaeen A, AbdullahH ‎, Abdulhussain L, MohammedW ‎, Hassan A. Different Concentrations of Honey’s Antimicrobial Activity Against ‎Staphylococcus Aureus by HPLC Determination. cuesj [Internet]. 25Sep.2022 [cited 28Nov.2022];6(2):112-8. Available from: https://journals.cihanuniversity.edu.iq/index.php/cuesj/article/view/712
Section
Research Article