The Prevalence of Microorganisms and Antibiotics Activity Tests of Natural and Industrial Products in Surgical Site Infections in Referral Hospitals in Erbil City, Kurdistan Region – Iraq

  • Raid D. Thanoon Department of Medical Biochemical Analysis , Cihan University-Erbil, Kurdistan Region, Iraq.
  • Yara M. Mohammed Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq.
Keywords: Surgical site infections, bacteria isolated from wounds, antimicrobial resistance, pus, plant extract

Abstract

Background and objectives: To study the prevalence of microorganisms and antibiotics activity tests of natural and industrial products, SSIs were found in referral hospitals in Erbil, Iraq's Kurdistan province. MethodsPus samples were collected from 29 surgical patients using sterile swabs and primed for culture collection. Disc diffusion method was used to determine the bacterial activity of three types of plants (cinnamon, ginger, and radish) against three bacterial species including Staphylococcus aureus, Proteus spp., and S.epidermidis. Result: Overall 29 pus samples were processed for culture testing. One gram negative bacterium (7.14%) and thirteen gram positive cocci (92.85%) were isolated from the 14 bacteria. The three most potent isolates were found to be resistant to the variable of tested antibiotics, while they showed any sensitivity to the tested plant extracts. Cinnamon, ginger, and radish extracts were discovered to be among the few variable effective plant extracts against the tested pathogenic bacteria isolates. Conclusion: The data collected from this research showed a better way of explanation of the microorganism’s etiology of surgical site infections (SSIs) in hospitals where they might have epidemiological and healing insinuations.

Keywords: Surgical Site Infections, Bacteria isolated from wounds, antimicrobial resistance, pus, plant extract.

Downloads

Download data is not yet available.

References

T. Anggrahita, A. Wardhana and G. Sudjatmiko. Chlorhexidinealcohol versus povidone-iodine as preoperative skin preparation to prevent surgical site infection:A meta-analysis. Medical Journal of Indonesia, vol. 26, no. 1, pp. 54-61, 2017.

K. S. Bhaskarrao, R. Vaishali and V. J. Katkar. Study of bacteriological profile of post-operative wound infections in

obstetrics and gynaecological surgeries in a tertiary care hospital. National Journal of Integrated Research in Medicine, vol. 9, no. 2, pp. 77-83, 2018.

B. Krismer, C. Weidenmaier, A. Zipperer and A. Peschel. The commensal lifestyle of Staphylococcus aureus and its interactions with the nasal microbiota. Nature Reviews Microbiology, vol. 15, no. 11, pp. 675-687, 2017.

K. Y. Le, M. D. Park and M. Otto. Immune evasion mechanisms of Staphylococcus epidermidis biofilm infection. Frontiers in Microbiology, vol. 9, p. 359, 2018.

X. X. Ma, E. H. Wang, Y. Liu and E. J. Luo. Antibiotic susceptibility of coagulase-negative staphylococci (CoNS): Emergence of teicoplanin-non-susceptible CoNS strains with inducible resistance to vancomycin. Journal of Medical Microbiology, vol. 60, no. 11, pp. 1661-1668, 2011.

S. Saima, M. U. Arshad, F. Saeed, R. S. Ahmad, A. Imran and T. Tufail. Nutritional characterization of cinnamon and turmeric with special reference to their antioxidant profile. International Journal of Biosciences, vol. 15, no. 4, pp. 178-187, 2019.

Y. Wang, W. Liu, L. Xu, Y. Wang, Y. Chen, X. Luo, M. Tang and L. Liu. Development of SNP markers based on transcriptome sequences and their application in germplasm identification in radish (Raphanus sativus L.). Molecular Breeding, vol. 37, no. 3, p. 26, 2017.

M. H. Shahrajabian, W. Sun and Q. Cheng. Pharmacological uses and health benefits of ginger (Zingiber officinale) in traditional Asian and ancient Chinese medicine, and modern practice. Notulae Scientia Biologicae, vol. 11, no. 3, pp. 309-319, 2019.

V. Negi. Bacteriological profile of surgical site infections and their antibiogram:A study from resource constrained rural setting of Uttarakhand state, India. Journal of Clinical and Diagnostic Research, vol. 9, no. 10, pp. DC17-DC20, 2015.

B. Tammy L. Staphylococci and other Catalase Positive Cocci that Grow Aerobically. 8th ed. Washington, DC: ASM Press, pp. 384-404, 2003.

M. Olson, M. O’Connor and M. L. Schwartz. Surgical wound infections. A 5-year prospective study of 20,193 wounds at the Minneapolis VA medical center. Journal of Urology, vol. 132, no. 2, pp. 421-421, 1984.

D. I. Torres, M. V. Miranda and V. C. Dall’ Orto. One-pot preparation of SBP-PANI-PAA-ethylene glycol diglycidyl ether sensor for electrochemical detection of H2O2. Sensors and Actuators B: Chemical, vol. 239, pp. 1016-1025, 2017.

Kwon, X. Liu, E. G. Choi, J. Y. Lee, S. Y. Choi, J. Y. Kim, L. Wang, S. J. Park, B. Kim, Y. A. Lee, J. J. Kim, N. Y. Kang and Y. T. Chang. Development of a universal fluorescent probe for gram‐positive bacteria. Angewandte Chemie, vol. 58, no. 25, pp. 8426-8431, 2019.

A. Naha, C. Sahu, P. Datta, S. Banerjee, J. Konar and S. Pal. Staphylococcus haemolyticus under reported because of false positive slide coagulase test: Implications from two case reports. Journal of Evolution of Medical and Dental Sciences, vol. 4, no. 21, pp. 3731-3735, 2015.

N. Gayathree and D. Shrinivas.A study on surgical site infections caused by Staphylococcus aureus with a special search for methicillin-resistant isolates. Journal of Clinical and Diagnostic Research, vol. 5, no. 3, pp. 502-508, 2011.

I. Kamrul, R. A. Afroz, K. M. Murad and K. M. Shahidul. Antimicrobial activity of ginger (Zingiber officinale) extracts against food-borne pathogenic bacteria. International Journal of Science, Environment and Technology, vol. 3, no. 3, pp. 867-871, 2014.

M. D. Soković, J. Glamočlija, A. Ćirić, D. Grubišić, D. Stojković and M. Ristić. Antimicrobial activity of essential oils isolated from different parts of endemic plant Portenschlagiella ramosissima Tutin. Journal of Essential Oil Research, vol. 20, no. 4, pp. 369-372, 2008.

D. Stojković, M. Smiljković, M. Nikolić, J. Živković and M. Soković. Sensitivity of multiresistant bacteria and methicillinresistant Staphylococcus aureus to ethanolic root extract of Raphanus sativus. Lekovite sirovine, vol. 38, pp. 35-38, 2018.

S. Waty, D. Suryanto and Yurnaliza. Antibacterial activity of cinnamon ethanol extract (Cinnamomum burmannii) and its application as a mouthwash to inhibitstreptococcusgrowth. IOP Conference Series: Earth and Environmental Science, vol. 130, p. 012049, 2018.

D. K. Kumar. Surgical site infection in clean, clean-contaminated and contaminated cases. Journal of Medical Science and Clinical Research, vol. 4, no. 12, pp. 14981-14986, 2016.

S. Shrestha, R. Shrestha, B. Shrestha and A. Dongol. Incidence and risk factors of surgical site infections in Kathmandu university hospital, Kavre, Nepal. Kathmandu University Medical Journal, vol. 14, no. 54, pp. 107-111, 2016.

J. Segreti, J. Parvizi, E. Berbari, P. Ricks and S. I. Berríos-Torres. Introduction to the centers for disease control and prevention and healthcare infection control practices advisory committee guideline for prevention of surgical site infection: prosthetic joint arthroplasty section. Surgical Infections, vol. 18, no. 4, pp. 394-400, 2017.

H. Guan, W. Dong, Y. Lu, M. Jiang, D. Zhang, Y. Aobuliaximu, J. Dong, Y. Niu, Y. Liu, B. Guan, J. Tang and S. Lu. Distribution and antibiotic resistance patterns of pathogenic bacteria in patients with chronic cutaneous wounds in China. Frontiers in Medicine, vol. 8, p. 609584, 2021.

P. Neopane, H. P. Nepal, R. Shrestha, O. Uehara and Y. Abiko. In vitro biofilm formation by Staphylococcus aureus isolated from wounds of hospital-admitted patients and their association with antimicrobial resistance. International Journal of General Medicine, vol. 11, pp. 25-32, 2018.

X. Zhu, X. Sun, Y. Zeng, W. Feng, J. Li, J. Zeng and Y. Zeng. Can nasal Staphylococcus aureus screening and decolonization prior to elective total joint arthroplasty reduce surgical site and prosthesis-related infections? A systematic review and metaanalysis. Journal of Orthopaedic Surgery and Research, vol. 15, no. 1, p. 60, 2020.

B. C. G. Slingerland, M. C. Vos, W. Bras, R. F. Kornelisse, D. De Coninck, A. van Belkum, I. K. M. Reiss, W. H. F. Goessens, C. H. W. Klaassen and N. J. Verkaik. Whole-genome sequencing to explore nosocomial transmission and virulence in neonatal methicillin-susceptible Staphylococcus aureus bacteremia. Antimicrobial Resistance and Infection Control, vol. 9, no. 1, p. 39, 2020.

L. Ying, X. Zhe, Y. Zhou, S. Juan and M. Lin. Characterization of community-associated Staphylococcus aureus from skin and softtissue infections:A multicenter study in China. Emerging Microbes and Infections, vol. 5, no. 1, pp. 1-11, 2016.

L. Radlinski, S. E. Rowe, L. B. Kartchner, R. Maile, B. A. Cairns, N. P. Vitko, C. J. Gode, A. M. Lachiewicz, M. C. Wolfgang and B. P. Conlon.Pseudomonas aeruginosa exoproducts determine antibiotic efficacy against Staphylococcus aureus. PLOS Biology, vol. 15, no. 11, p. e2003981, 2017.

M. H. Chowdhury, T. Debnath, S. Bhowmik and T. Islam. Presence of multidrug-resistant bacteria on mobile phones of healthcare workers accelerates the spread of nosocomial infection and regarded as a threat to public health in Bangladesh. Journal of Microscopy and Ultrastructure, vol. 6, no. 3, p. 165, 2018.

R. Chabi and H. Momtaz. Virulence factors and antibiotic resistance properties of the Staphylococcus epidermidis strains isolated from hospital infections in Ahvaz, Iran. Tropical Medicine and Health, vol. 47, no. 1, p. 47, 2019.

W. R. P. Wijesooriya, D. N. Kotsanas, T. M. Korman and M. Graham. Teicoplanin non-susceptible coagulase-negative staphylococci in a large Australian healthcare network: Implications for treatment with vancomycin. Sri Lankan Journal of Infectious Diseases, vol. 7, no. 1, p. 10, 2017.

M. A. Mohammed, A. M. J. Abd Al-Abbas and A. M. Alsamak. Distribution of OatA alleles detected by a new designed primer in bacteria isolated from eye infections in Basrah governorate/Iraq. Annals of the Romanian Society for Cell Biology, vol. 25, no. 3, pp. 8258-8277, 2021.

D. Girlich, R. A. Bonnin, L. Dortet and T. Naas. Genetics of acquired antibiotic resistance genes in Proteus spp. Frontiers in Microbiology, vol. 11, p. 256, 2020.

S. Haq, S. Dildar, M. B. Ali, A. Mezni and A. Hedfi. Antimicrobial and antioxidant properties of biosynthesized of NiO nanoparticles using Raphanus sativus (R. sativus) extract. Materials Research Express, vol. 8, no. 5, p. 55006, 2021.

Ç. Güceyü, G. Goncagül, E. Günaydin and P. Akpinar. Zencefil’in antibakteriyal etkisi (antibacterial effect of Zingiber officinale(Ginger)). Etlik Veteriner Mikrobiyoloji Dergisi, vol. 30, no. 1, pp. 44-50, 2019.

Y. Zhang, X. Liu, Y. Wang, P. Jiang and S. Quek. Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus. Food Control, vol. 59, pp. 282-289, 2016.

E. Julianti, K. K. Rajah and I. Fidrianny. Antibacterial activity of ethanolic extract of cinnamon bark, honey, and their combination effects against acne-causing bacteria. Scientia Pharmaceutica, vol. 85, no. 2, p. 19, 2017.

K. I. Al-Zubaidy. Cinnamon’s antibacterial activity on the bacterial isolates from urinary tract infections. World Journal of Pharmaceutical Research, vol. 6, no. 17, pp. 81-91, 2017

Published
2021-08-10
How to Cite
1.
Thanoon R, Mohammed Y. The Prevalence of Microorganisms and Antibiotics Activity Tests of Natural and Industrial Products in Surgical Site Infections in Referral Hospitals in Erbil City, Kurdistan Region – Iraq. cuesj [Internet]. 10Aug.2021 [cited 16Apr.2024];5(2):6-2. Available from: https://journals.cihanuniversity.edu.iq/index.php/cuesj/article/view/398
Section
Research Article