The Preventive Effects of Urtica dioica ‎Extract and Nanoparticle on Oxidative Stress ‎and Lipid Profile in Hyperlipidemic Male ‎Rats

Keywords: Hyperlipidemia, Antioxidant, Urtica dioica, Silver nanoparticle, Cardiovascular disease

Abstract

Cardiovascular diseases are the major factor in human premature death and disability and they are becoming more common worldwide. Individual atherosclerosis risk factors such obesity, hypertension, hyperlipidemia, diabetes, and smoking all lead to an increase in oxidative stress. As a result, oxidative stress is thought to be a major factor in the development of atherosclerosis. Heart disease risk factors that have been well-documented include elevated blood lipid levels. Significant gaps in the treatment of CVDs still exist, in addition to making healthy lifestyle changes and using lipid-lowering medications as part of primary CVD prevention and therapy regimens. Natural extracts are a rich source of biomolecules that can be used as complex reagents for the biogenic synthesis of silver nanoparticles as well as antioxidant medicines or dietary supplements. The current study estimates the anti-oxidant and protective activity of Urica dioica 200mg/kg extract water and ethanol with its silver nanoparticles 15mg/kg on lipid profile in induced hyperlipidemia rats feeding on high fat diet then evaluate lipid profile and MDA, SOD and GSH of the serum after 40 days, also characterization of AgNPs done by UV-Vis absorption, FTIR, XRD and SEM. The result demonstrates that the successful synthesis of NPs and Urtica dioica has a favorable impact on lipid profile and can function as a good antioxidant that can be employed as a protective agent against rising lipids and oxidative stress. However, further research studies need to investigate the safety and activity of different dose of AgNPS of Urtica dioica.

Downloads

Download data is not yet available.

Author Biographies

Banw A. Othman, Department of Pharmacognosy, College of pharmacy, Hawler Medical University, Erbil, Kurdistan Region, Iraq

Banw A. Othman is a teaching assistant at department of pharmacognosy, collage of pharmacy, Hawler medical University. She got a B.Sc. degree in biology collage of science, Salahaddin University. She is M.Sc. student at the department of biology, collage of education, Salahaddin University. Her research interests are in microbiome in gut, antioxidant , antiaging.

Nadir M. Nanakali, Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq

Nadir M. Nanakali is a prof. at the department of biology, collage of education, Salahaddin University. He got a B.Sc. degree in biology collage of education, Salahaddin University.an M.Sc. degree in physiology, collage of education, Salahaddin University. another M.Sc. degree in physiology, collage of education, Baghdad University. Ph.D. degree in physiology, collage of education, Baghdad University. His research interests are in diabetic, hyperlipidemia, antioxidant, nanoparticles.

References

J. Miao, X. Zang, X. Cui and J. Zhang. Autophagy, hyperlipidemia, and atherosclerosis. Advances in Experimental Medicine and Biology, vol. 1207, pp. 237-264, 2020.

N. He and H. Ye. Exercise and hyperlipidemia. Advances in Experimental Medicine and Biology, vol. 1228, pp. 79-90, 2020.

R. Stocker and J. F. Keaney, Jr. Role of oxidative modifications in atherosclerosis. Physiological Reviews, vol. 84, no. 4, pp. 1381-1478, 2004.

R. M. Pérez Gutiérrez, A. Muñiz-Ramirez, A. H. Garcia-Campoy and J. M. Mota Flores. Evaluation of the antidiabetic potential of extracts of Urtica dioica, apium graveolens, and Zingiber officinale in mice, Zebrafish, and pancreatic β-Cell. Plants (Basel), vol. 10, no. 7, p. 1438, 2021.

M. A. Mayer, C. Höcht, A. Puyó and C. A. Taira. Recent advances in obesity pharmacotherapy. Current Clinical Pharmacology, vol. 4, no. 1, pp. 53-61, 2009.

J. Flieger, W. Franus, R. Panek, M. Szymańska-Chargot, W. Flieger, M. Flieger and P. Kołodziej. Green synthesis of silver nanoparticles using natural extracts with Proven antioxidant activity. Molecules, vol. 26, no. 16, p. 49886, 2021.

F. Mahmoudi, F. Mahmoudi, K. H. Gollo and M. M. Amini. Biosynthesis of novel silver nanoparticles using eryngium thyrsoideum boiss extract and comparison of their antidiabetic activity with chemical synthesized silver nanoparticles in diabetic rats. Biological Trace Element Research, vol. 199, no. 5, pp. 1967-1978, 2021.

G. Avci, E. Kupeli, A. Eryavuz, E. Yesilada and I. Kucukkurt. Antihypercholesterolaemic and antioxidant activity assessment of some plants used as remedy in Turkish folk medicine. Journal of Ethnopharmacology, vol. 107, no. 3, pp. 418-423, 2006.

F. Namazi, T. Shomali, P. Taghikhani and S. Nazifi. Protective effect of Urtica dioica leaf hydro alcoholic extract against experimentally-induced atherosclerosis in rats. Avicenna J Phytomed, vol. 8, no. 3, pp. 254-262, 2018.

H. V. Annegowda, R. Bhat, L. Min-Tze, A. A. Karim and S. M. Mansor. Influence of sonication treatments and extraction solvents on the phenolics and antioxidants in star fruits. Journal of Food Science and Technology, vol. 49, no. 4, pp. 510-514, 2012.

A. Jadou and A. W. Al-Shahwany. Biogenic synthesis and characterization of silver nanoparticles using some medical plants and evaluation of their antibacterial and toxicity potential. Journal of AOAC International, vol. 101, no. 6, pp. 1905-1912, 2018.

S. Mojarradgandoukmolla, N. M. Q. Nanakali and A. Abjabbar. Hypolipidemic and anti-oxidative activities of Terminalia arjuna barks against induced hyperlipidemic albino rats. Plant Archives, vol. 21, pp. 2082-2086, 2021.

P. B. E. Kedi, F. E. Meva, L. Kotsedi, E. L. Nguemfo, C. B. Zangueu, A. A. Ntoumba, H. E. A. Mohamed, A. B. Dongmo and M. Maaza. Eco-friendly synthesis, characterization, in vitro and in vivo antiinflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract. International Journal of Nanomedicine, vol. 13, pp. 8537-8548, 2018.

S. J. P. Jacob, V. L. S. Prasad, S. Sivasankar and P. Muralidharan. Biosynthesis of silver nanoparticles using dried fruit extract of Ficus carica Screening for its anticancer activity and toxicity in animal models. Food and Chemical Toxicology, vol. 109, no. Pt 2, pp. 951-956, 2017.

M. M. Kadhim, A.N. Aldujaili and M.H. Homady. Assessment of hepatoprotective role of phenolic extract of Urtica dioica and silver nanoparticles in male rat induced by carbon tetra-chloride. Rasayan Journal of Chemistry, vol. 10, p. 305-688, 2017.

S. Mojarrad, N. M. Q. Nanakali and M. Q. Khursheed. Hypolipidemic Efficacy of Omega-3 Fatty Acids in Comparison with Rosuvastatin in Induced Hyperlipidemic Albino Rats. In: International Journal of Pharmaceutical and Phytopharmacological Research, vol. 10, no. 5, pp. 170-178, 2020.

Q. Y. Peng, Y. M. Wang, C. X. Chen, Y. Zou, L. N. Zhang, S. Y. Deng and Y. H. Ai Inhibiting the CD38/cADPR pathway protected rats against sepsis associated brain injury. Brain Research, vol. 1678, pp. 56-63, 2018.

W. T. Friedewald, R. I. Levy and D. S. Fredrickson. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry, vol. 18, no. 6, pp. 499-502, 1972.

D. Armbruster. Optimized kinetic glutamate dehydrogenase assay adapted to the Cobas Bio centrifugal analyzer. Clinical Chemistry, vol. 33, no. 9, pp. 1673-1674, 1987.

M. Devaki, R. Nirupama and H. N. Yajurvedi. Chronic stressinduced oxidative damage and hyperlipidemia are accompaniedby atherosclerotic development in rats. Stress, vol. 16, no. 2, pp. 233-243, 2013.

A. J. Alpert and H. F. Gilbert. Detection of oxidized and reduced glutathione with a recycling postcolumn reaction. Analytical Biochemistry, vol. 144, no. 2, pp. 553-562, 1985.

I. Augspole, M. Duma, B. Ozola and I. Cinkmanis. Phenolic profile of fresh and frozen nettle, goutweed, dandelion and chickweed leaves. 11th Baltic Conference on Food Science and Technology Food Science and Technology in a Changing World, p. 36-39, 2017.

A. L. B. Zeni, T. D. Moreira, A. P. Dalmagro, A. Camargo, L. A. Bini, E. L. Simionatto and D. R. Scharf. Evaluation of phenolic compounds and lipid-lowering effect of Morus nigra leaves extract. Anais Academia Brasileira Ciencias, vol. 89, no. 4, pp. 2805-2815, 2017.

R. Amarowicz and R. B. Pegg. The potential protective effects of phenolic compounds against low-density lipoprotein oxidation. Current Pharmaceutical Design, vol. 23, no. 19, pp. 2754-2766, 2017.

C. F. Daher, K. G. Baroody and G. M. Baroody. Effect of Urtica dioica extract intake upon blood lipid profile in the rats. Fitoterapia, vol. 77, no. 3, pp. 183-188, 2006.

A. D. Sniderman, J. Bergeron and J. Frohlich. Apolipoprotein B versus lipoprotein lipids: Vital lessons from the AFCAPS/TexCAPS trial. CMAJ, vol. 164, no. 1, pp. 44-47, 2001.

M. Nassiri-Asl, F. Zamansoltani, E. Abbasi, M. M. Daneshi and A. A. Zangivand. Effects of Urtica dioica extract on lipid profile in hypercholesterolemic rats. Zhong Xi Yi Jie He Xue Bao, vol. 7, no. 5, pp. 428-433, 2009.

Z. Kukric, L. Topalić-Trivunović, S. B. Matoš, B. Kukavica, S. S. Kukavica, M. M. Boroja and A. V. Savić. Characterization of antioxidant and antimicrobial activities of nettle leaves (Urtica dioica L.). Acta Periodica Technologica, vol. 43, pp. 257-272, 2012.

O. Rau, M. Wurglics, T. Dingermann, M. Abdel-Tawab and M. Schubert-Zsilavecz. Screening of herbal extracts for activation of the human peroxisome proliferator-activated receptor. Pharmazie, vol. 61, no. 11, pp. 952-956, 2006.

S. C. Choe, H. S. Kim, T. S. Jeong, S. H. Bok and Y. B. Park. Naringin has an antiatherogenic effect with the inhibition of intercellular adhesion molecule-1 in hypercholesterolemic rabbits. Journal of Cardiovascular Pharmacology, vol. 38, no. 6, pp. 947-955, 2001.

M. Kanter, I. Meral, S. Dede, H. Gunduz, M. Cemek, H. Ozbek and I. Uygan. Effects of Nigella sativa L. and Urtica dioica L. on lipid peroxidation, antioxidant enzyme systems and some liver enzymes in CCl4-treated rats. Journal of Veterinary Medicine Series A, vol. 50, no. 5, pp. 264-268, 2003.

M. K. Türkdoğan, H. Ozbek, Z. Yener, I. Tuncer, I. Uygan and E. Ceylan. The role of Urtica dioica and Nigella sativa in the prevention of carbon tetrachloride-induced hepatotoxicity in rats. Phytotherapy Research, vol. 17, no. 8, pp. 942-946, 2003.

L. Yang, H. Kuang, W. Zhang, Z. P. Aguilar, H. Wei and H. Xu. Comparisons of the biodistribution and toxicological examinations after repeated intravenous administration of silver and gold nanoparticles in mice. Scientific Reports, vol. 7, no. 1, p. 3303, 2017.

W. H. De Jong, L. T. Van Der Ven, A. Sleijffers, M. V. D. Park, E. H. J. Jansen, H. V. Loveren and R. J. Vandebriel. Systemic and immunotoxicity of silver nanoparticles in an intravenous 28 days repeated dose toxicity study in rats. Biomaterials, vol. 34, no. 33, pp. 8333-8343, 2013.

P. Maneewattanapinyo, W. Banlunara, C. Thammacharoen, S. Ekgasit and T. Kaewamatawong. An evaluation of acute toxicity of colloidal silver nanoparticles. The Journal of Veterinary Medical Science, vol. 73, no. 11, pp. 1417-1423, 2011.

D. K. Tiwari, T. Jin and J. Behari. Dose-dependent in-vivo toxicity assessment of silver nanoparticle in Wistar rats. Toxicology Mechanisms and Methods, vol. 21, no. 1, pp. 13-24, 2011.

B. Farzami, D. Ahmadvand, S. Vardasbi, F. J. Majin and S. Khaghani. Induction of insulin secretion by a component of Urtica dioica leave extract in perifused islets of langerhans and its in vivo effects in normal and streptozotocin diabetic rats. Journal of Ethnopharmacology, vol. 89, no. 1, pp. 47-53, 2003.

H. Sovová, L. Opletal, M. Sajfrtová and M. Bártlová. Supercritical fluid extraction of cynaropicrin and 20-hydroxyecdysone from Leuzea carthamoides DC. Journal of Separation Science, vol. 31, no. 8, pp. 1387-1392, 2008.

H. Li, Y. Yao and L. Li. Coumarins as potential antidiabetic agents. Journal of Pharmacy and Pharmacology, vol. 69, no. 10, pp. 1253-1264, 2017.

S. Das, S. Vasisht, N. Das, L. M. Srivastava, K. Dubey and G. Watal. Correlation between total antioxidant status and lipid peroxidation in hypercholesterolemia. Current Science Bangalore, vol. 78, no. 4, pp. 486-486, 2000.

L. P. Stanojević. The effect of extraction techniques on yield, extraction kinetics, and antioxidant activity of aqueous-methanolic extracts from nettle (Urtica dioica L.) leaves. Separation Science and Technology, vol. 51, no. 11, pp. 1817-1829, 2016.

D. V. Ratnam, D. D. Ankola, V. Bhardwaj, D. K. Sahana and M. N. Kumar. Role of antioxidants in prophylaxis and therapy: A pharmaceutical perspective. Journal of Controlled Release, vol. 113, no. 3, pp. 189-207, 2006.

U. S. Harput, I. Saracoglu and Y. Ogihara. Stimulation of lymphocyte proliferation and inhibition of nitric oxide production by aqueous Urtica dioica extract. Phytotherapy Research, vol. 19, no. 4, pp. 346-8, 2005.

R. Bisht, B. C. Joshi, A. N. Kalia and A. Prakash. Antioxidantrich fraction of Urtica dioica mediated rescue of striatal mitooxidative damage in MPTP-induced behavioral, cellular, and neurochemical alterations in rats. Molecular Neurobiology, vol. 54, no. 7, pp. 5632-5645, 2017.

Published
2022-11-01
How to Cite
1.
Othman B, Nanakali N. The Preventive Effects of Urtica dioica ‎Extract and Nanoparticle on Oxidative Stress ‎and Lipid Profile in Hyperlipidemic Male ‎Rats. cuesj [Internet]. 1Nov.2022 [cited 28Nov.2022];6(2):125-32. Available from: https://journals.cihanuniversity.edu.iq/index.php/cuesj/article/view/756
Section
Research Article