Isolation and Identification of Some Pathogens of Terrestrial Snail Rumina decollata from Erbil Province, Kurdistan Region, Iraq

Authors

DOI:

https://doi.org/10.24086/cuesj.v10n1y2026.pp49-55

Keywords:

Land snails, Rumina decollate, bacteria, larval trematodes, Iraq

Abstract

Terrestrial snails are known to act as intermediate hosts for parasites and carriers of various bacteria. The present study aimed to isolate and identify some bacteria and larval trematodes from the terrestrial snail Rumina decollata (Linnaeus, 1758) collected from different locations in Erbil Province, Kurdistan Region, Iraq. Snail specimens were processed using standard laboratory techniques for bacteriological culture and parasitological examination. Several bacterial isolates were identified based on morphological and biochemical characteristics, including (Klebsiella pneumoniae, Citrobacter koseri [Citrobacter diversus], Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae, Acinetobacter baumannii, Citrobacter freundii, Stenotrophomonas maltophilia, Pseudomonas mendocina, Pantoea agglomerans [Enterobacter agglomerans], Acinetobacter calcoaceticus). In addition, larval trematodes of Lutztrema attenuatum were detected in a proportion of the examined snails. The results indicated that R. decollata harbors different bacterial species and larval trematodes, as the first investigation in Iraq. The presence of these organisms indicates that R. decollata may play a role in the transmission of bacterial and parasitic infections, which may pose risks to public and animal health, and contribute to a better understanding of the epidemiological significance of terrestrial snails in the region.

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Author Biographies

Zhylan M. Qader, Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq

Zhylan M. Qader studied Biology at the Department of Biology, College of science , Cihan University-Erbil, Kurdistan Region, Iraq. Her research interest is biology.

 

Shwan K. Bashê, Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq

Shwan K. Bashê is a lecturer at the Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq. His research interest is biology.

References

1. V. Prévot, K. Jordaens and T. Backeljau. “Predominance of a single phylogenetic species in colonization events among a sextet of decollate land snail, Rumina decollata (Mollusca: Pulmonata: Subulinidae), species”. Genome, vol. 57, no. 3, pp. 161-167, 2014. DOI: https://doi.org/10.1139/gen-2013-0187

2. N. M. Cardillo, M. Ercole, F. Fariña, M. Pasqualetti, Y. Loiza, M. Pérez, A. Bonboni and M. Ribicich. “Larval development of Aelurostrongylus abstrusus in experimentally infected Rumina decollata snails”. Veterinary Parasitology, vol. 251, pp. 50-55, 2018. DOI: https://doi.org/10.1016/j.vetpar.2018.01.002

3. R. H. Al-Qaisi and K. Farman. “New record of exotic land snail Rumina decollate (Linnaeus, 1758) in Iraq”. Communications Faculty of Sciences University of Ankara Series C Biology, vol. 32, no. 1, pp. 54-58, 2023. DOI: https://doi.org/10.53447/communc.1202072

4. A. I. Rau, A. A. Beltramino, E. N. Serniotti, J. Pizá, J. D. Caffetti and R. E. Vogler. “The exotic snail Rumina decollata (Linnaeus, 1758)(Gastropoda, Achatinidae, Rumininae) in Argentina: New records, range extension, and areas of origin of Argentine populations”. Check List, vol. 18, no. 1, pp. 91-102, 2022. DOI: https://doi.org/10.15560/18.1.91

5. S. K. Bashê and S. E. Al-Qassab. “Morphological and molecular identification of some terrestrial snails: First report”. Zanco Journal of Pure and Applied Sciences, vol. 36, no. 1, pp. 94-104, 2024. DOI: https://doi.org/10.21271/zjpas.36.1.9

6. I. N. Ghulam. “First record of Rumina Decollata (Linnaeus, 1758), a terrestrial snail, in the middle euphrates region, Iraq”. Journal of Natural Science Biology and Medicine, vol. 16, no. 1, p. 166, 2025.

7. K. Apostolou, C. Radea, A. Meziti and K. A. Kormas. “Bacterial diversity associated with terrestrial and aquatic snails”. Microorganisms, vol. 13, no. 1, p. 8, 2024. DOI: https://doi.org/10.3390/microorganisms13010008

8. R. Mc Donnell, R. Santangelo, T. Paine and M. Hoddle. “The feeding behaviour of Rumina decollata (Subulinidae: Gastropoda) raises questions about its efficacy as a biological control agent for Cornu aspersum (Helicidae: Gastropoda)”. Biocontrol Science and Technology, vol. 26, no. 3, pp. 331-336, 2016. DOI: https://doi.org/10.1080/09583157.2015.1111298

9. M. Matamoros. “Los moluscos fitófagos en la agricultura cubana”. Agricultura Orgánica, vol. 20, no. 2, pp. 9-13, 2014.

10. S. M. Said and S. M. Ali. “Effects of bacteria Bacillus thuringiensis (Bt) on the diges‑tive system of the land snail Eobania vermiculata”. International Journal of Ecotoxicology and Ecobiology, vol. 3, no. 1, pp. 17-21, 2018.

11. M. C. T. Landal, R. P. Bach, S. R. Gomes, M. Botton and M. A. C. Zawadneak. “Gastrópodes terrestres como pragas do cultivo de Fragaria x ananassa no sul do Brasil: Identificação morfológica”. Ciência Rural, vol. 49, p. e20180444, 2019. DOI: https://doi.org/10.1590/0103-8478cr20180444

12. D. Kowalczyk-Pecka and A. Puchalski. “Potential interaction between the Cepaea nemoralis wild snail and Citrobacter spp. Bacteria”. Vet Med, vol. 64, no. 6, pp. 786-790, 2008.

13. C. Gérard, Y. De Tombeur, M. Dahirel and A. Ansart. “Land snails can trap trematode cercariae in their shell: Encapsulation as a general response against parasites?”. Parasite, vol. 30, p. 1, 2023. DOI: https://doi.org/10.1051/parasite/2023001

14. T. A. Dallas, A. L. Laine and O. Ovaskainen. “Detecting parasite associations within multi-species host and parasite communities”. Proceedings of the Royal Society B, vol. 286, no. 1912, p. 20191109, 2019. DOI: https://doi.org/10.1098/rspb.2019.1109

15. T. Waki, M. Nakao, K. Hayashi, H. Ikezawa and N. Tsutumi. “Molecular and morphological discrimination of dicrocoeliid larvae (Trematoda: Digenea) from terrestrial mollusks in Japan”. Journal of Parasitology, vol. 104, no. 6, pp. 660-670, 2018. DOI: https://doi.org/10.1645/18-24

16. V. V. Tkach, T. J. Achatz, J. Hildebrand and S. E. Greiman. “Convoluted history and confusing morphology: Molecular phylogenetic analysis of dicrocoeliids reveals true systematic position of the Anenterotrematidae Yamaguti, 1958 (Platyhelminthes, Digenea)”. Parasitology International, vol. 67, no. 4, pp. 501-508, 2018. DOI: https://doi.org/10.1016/j.parint.2018.04.009

17. H. Furusawa and T. Waki. “A description of a new species of the genus Brachydistomum (trematode, dicrocoeliidae) from the eurasian tree sparrow passer montanus (linnaeus) (passeriformes) in Japan, with a report on the first intermediate host”. Systematic Parasitology, vol. 101, no. 2, p. 22, 2024. DOI: https://doi.org/10.1007/s11230-024-10148-1

18. P. Segade, C. Crespo, N. García, J. García-Estévez, C. Arias and R. Iglesias. “Brachylaima aspersae n. Sp.(Digenea: Brachylaimidae) infecting farmed snails in NW Spain: Morphology, life cycle, pathology, and implications for heliciculture”. Veterinary Parasitology, vol. 175, no. 3-4, pp. 273-286, 2011. DOI: https://doi.org/10.1016/j.vetpar.2010.10.026

19. J. Hildebrand, J. Sitko, G. Zaleśny, W. Jeżewski and Z. Laskowski. “Molecular characteristics of representatives of the genus Brachylecithum shtrom, 1940 (Digenea, Dicrocoeliidae) with comments on life cycle and host specificity”. Parasitology Research, vol. 115, no. 4, pp. 1417-1425, 2016. DOI: https://doi.org/10.1007/s00436-015-4875-3

20. G. P. P. De León and D. I. Hernández-Mena. “Testing the higher level phylogenetic classification of Digenea (Platyhelminthes, Trematoda) based on nuclear rDNA sequences before entering the age of the ‘next-generation’ tree of life”. Journal of Helminthology, vol. 93, no. 3, pp. 260-276, 2019. DOI: https://doi.org/10.1017/S0022149X19000191

21. MolluscaBase. “ MolluscaBase. Available from: https://www.molluscabase.org [Last accessed on 2025 Dec 12].

22. D. G. Herbert. “The Introduced Terrestrial Mollusca of South Africa”. South African National Biodiversity Institute, South Africa, 2010.

23. V. Prevot, T. Backeljau and K. Jordaens. “Morphometric evaluation of DNA-based cryptic taxa in the terrestrial decollate snail genus Rumina”. Journal of Molluscan Studies, vol. 81, no. 2, pp. 223-232, 2015. DOI: https://doi.org/10.1093/mollus/eyu080

24. F. W. Welter-Schultes. European Non-Marine Molluscs, a Guide for Species Identification: Bestimmungsbuch Für Europäische Landund Süsswassermollusken. Planet Poster Editions, Germany, 2012.

25. M. A. Dar, K. D. Pawar, J. P. Jadhav and R. S. Pandit. “Isolation of cellulolytic bacteria from the gastro-intestinal tract of Achatina fulica (Gastropoda: Pulmonata) and their evaluation for cellulose biodegradation”. International Biodeterioration and Biodegradation, vol. 98, pp. 73-80, 2015. DOI: https://doi.org/10.1016/j.ibiod.2014.11.016

26. B. Chalifour and J. Li. “Characterization of the gut microbiome in wild rocky mountainsnails (Oreohelix strigosa)”. Animal microbiome, vol. 3, no. 1, p. 49, 2021. DOI: https://doi.org/10.1186/s42523-021-00111-6

27. R. Atlas. “Handbook of Microbiological Media”. United States: CRC Press, 2010. DOI: https://doi.org/10.1201/EBK1439804063

28. G. Funke, D. Monnet, C. DeBernardis, A. Von Graevenitz and J. Freney. “Evaluation of the VITEK 2 system for rapid identification of medically relevant gram-negative rods”. Journal of Clinical Microbiology, vol. 36, no. 7, pp. 1948-1952, 1998. DOI: https://doi.org/10.1128/JCM.36.7.1948-1952.1998

29. G. L. Pinheiro, R. F. Correa, R. S. Cunha, A.M. Cardoso, C. Chaia, M. M. Clementino, E. S. Garcia, De Souza, W. and Frases, S. “Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica”. Frontiers in Microbiology, vol. 6, p. 860, 2015. DOI: https://doi.org/10.3389/fmicb.2015.00860

30. G. C. Onyishi, I. O. Aguzie, J. O. Okoro, C. D. Nwani, N. Ezenwaji, O. N. Stanley and F. Okafor. “Terrestrial snail fauna and associated helminth parasites in a tropical semi-Urban Zone, Enugu State, Nigeria”. Pakistan Journal of Zoology, vol. 50, no. 3, p. 1080, 2018. DOI: https://doi.org/10.17582/journal.pjz/2018.50.3.1079.1085

31. P. Wiroonpan, T. Chontananarth and W. Purivirojkul. “Cercarial trematodes in freshwater snails from Bangkok, Thailand: Prevalence, morphological and molecular studies and human parasite perspective”. Parasitology, vol. 148, no. 3, pp. 366-383, 2021. DOI: https://doi.org/10.1017/S0031182020002073

32. M. N. Tanyitiku. “Nutritious food and public health risks: Insights on foodborne pathogens in edible land snails: A thesis submitted in partial fulfilment of the requirements for the degree of doctor of philosophy at Lincoln University”. Lincoln University, London, 2022.

33. L. P. Caullan, G. G. Vila, E. A. Angulo, A. Calvo, J. A. Marcelo and M. A. C. Torras. “Microbiota from Helix aspersa Müller in Barcelona Area (Spain)”. Advances in Microbiology, vol. 4, no. 10, pp. 604-608, 2014. DOI: https://doi.org/10.4236/aim.2014.410066

34. Y. Xu, L. Ni, H. Guan, D. Chen, S. Qin and L. Chen. “First report of potentially pathogenic Klebsiella pneumoniae from serotype K2 in Mollusk Tegillarca granosa and genetic diversity of Klebsiella pneumoniae in 14 species of edible aquatic animals”. Foods, vol. 11, no. 24, p. 4058, 2022. DOI: https://doi.org/10.3390/foods11244058

35. M. N. Tanyitiku, G. Nicholas, J. J. Sullivan, I. C. N. Petcheu and S. L. On. “Survival of Escherichia coli in edible land snails: Implications for heliciculture and public health”. Pathogens, vol. 13, no. 3, p. 204, 2024. DOI: https://doi.org/10.3390/pathogens13030204

36. S. M. Avery, A. Moore and M. L. Hutchison. “Fate of Escherichia coli originating from livestock faeces deposited directly onto pasture”. Letters in Applied Microbiology, vol. 38, no. 5, pp. 355-359, 2004. DOI: https://doi.org/10.1111/j.1472-765X.2004.01501.x

37. M. Y. Charrier, G. Fonty, B. Gaillard-Martinie, K. Ainouche and G. Andant. “Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helix pomatia and Cornu aspersum (Gastropoda: Pulmonata)”. Biological Research, vol. 39, no. 4, pp. 669-681, 2006. DOI: https://doi.org/10.4067/S0716-97602006000500010

38. A. M. Cardoso, J. J. Cavalcante, R. P. Vieira, J. L. Lima, M. A. Grieco, M. M. Clementino, A. T. Vasconcelos, E. S. Garcia, W. De Souza, R. M. Albano and O. B. Martins. “Gut bacterial communities in the giant land snail Achatina fulica and their modification by sugarcane-based diet”. PLoS One, vol. 7, no. 3, p. e33440, 2012. DOI: https://doi.org/10.1371/journal.pone.0033440

39. M. Ekperigin. “Preliminary studies of cellulase production by Acinetobacter anitratus and Branhamella sp”. African Journal of Biotechnology, vol. 6, no. 1, pp. 28-33, 2007.

40. E. Javadian, F. Forgani and F. Javadian. “Investigation of antimicrobial activity of Solanum nigrum and Saponaria officinalis extracts against Acinetobacter baumannii”. Gene, Cell and Tissue, vol. 6, no. 4, p. e93096, 2019. DOI: https://doi.org/10.5812/gct.93096

41. Z. Koleva, I. Dedov, J. Kizheva, R. Lipovanska, P. Moncheva and P. Hristova. “Lactic acid microflora of the gut of snail Cornu aspersum”. Biotechnology and Biotechnological Equipment, vol. 28, no. 4, pp. 627-634, 2014. DOI: https://doi.org/10.1080/13102818.2014.947071

42. M. A. Dar, K. D. Pawar and R. S. Pandit. “Gut microbiome analysis of snails: A biotechnological approach”. In: Organismal and Molecular Malacology/Ed. S. Ray. London: InTech Open, pp. 189-217, 2017.

43. E. Ivanova, M. Clausi, I. Sparacio and S. Spiridonov. “Preliminary data on the parasite survey of terrestrial gastropods of Sicily”. Russian Journal of Nematology, vol. 27, no. 1, pp. 37-45, 2019.

44. W. Tsukasa, T. Takano, H. Furusawa and M. Urabe. “First record of the natural first intermediate host of Lutztrema attenuatum (Dujardin, 1845), a cosmopolitan trematode of passerine birds”. J-stage, vol. 54, pp. 59-66, 2023.

45. M. Y. Manga-González, C. Gonzalez-Lanza, E. Cabanas and R. Campo. “Contributions to and review of dicrocoeliosis, with special reference to the intermediate hosts of Dicrocoelium dendriticum”. Parasitology, vol. 123, no. 7, pp. S91-S114, 2001. DOI: https://doi.org/10.1017/S0031182001008204

46. C. Coustau, B. Gourbal, D. Duval, T. Yoshino, C. Adema and G. Mitta. “Advances in gastropod immunity from the study of the interaction between the snail Biomphalaria glabrata and its parasites: A review of research progress over the last decade”. Fish and Shellfish Immunology, vol. 46, no. 1, pp. 5-16, 2015. DOI: https://doi.org/10.1016/j.fsi.2015.01.036

47. P. Heneberg, J. Sitko, M. Casero and I. Rząd. “New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)”. International Journal for Parasitology: Parasites and Wildlife, vol. 22, pp. 276-299, 2023. DOI: https://doi.org/10.1016/j.ijppaw.2023.11.004

48. R. F. Hechinger and K. D. Lafferty. “Host diversity begets parasite diversity: Bird final hosts and trematodes in snail intermediate hosts”. Proceedings of the Royal Society B: Biological Sciences, vol. 272, no. 1567, pp. 1059-1066, 2005. DOI: https://doi.org/10.1098/rspb.2005.3070

49. A. R. Butcher and D. I. Grove. “Description of the life-cycle stages of Brachylaima cribbi n. Sp.(Digenea: Brachylaimidae) derived from eggs recovered from human faeces in Australia”. Systematic Parasitology, vol. 49, no. 3, pp. 211-221, 2001. DOI: https://doi.org/10.1023/A:1010616920412

50. I. Ekin. “Introduction of Rumina decollata (Linnaeus, 1758) for pest snail anagement in citrus orchards in Türkiye: ecological, economic, and agricultural perspectives”. Agroecology and Sustainable Food Systems, vol. 49, no. 5, pp. 722-742, 2025. DOI: https://doi.org/10.1080/21683565.2024.2434664

51. A. Xie, Y. Zhang, M. F. Breed, X. An, H. Yao, Q. Huang, J. Suand X. Sun “Terrestrial invertebrate hosts of human pathogens in Urban ecosystems”. Eco Environment and Health, vol. 3, no. 3, pp. 369-380, 2024. DOI: https://doi.org/10.1016/j.eehl.2024.04.003

Published

2026-03-20

How to Cite

1.
Qader Z, Bashê S. Isolation and Identification of Some Pathogens of Terrestrial Snail Rumina decollata from Erbil Province, Kurdistan Region, Iraq: . Cihan U Erbil SCI J [Internet]. 2026 Mar. 20 [cited 2026 Jun. 23];10(1):49-55. Available from: https://journals.cihanuniversity.edu.iq/index.php/cuesj/article/view/1739

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