Correlation between Klebsiella Species’ Biofilm Formation and Antibiotic Resistance from Erbil Hospital Patients

Ali M.Hussein; Mohammed Issam Jameel; Naz R. Majeed; Aya A. Noori; Mina M. Mohammed; Washan N. Hamad; Adyan A. Mohammedsaeed

doi:10.24086/cuesj.v8n2y2024.pp56-64

Ali M.Hussein Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq https://orcid.org/0000-0001-5899-2047
Mohammed Issam Jameel Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq https://orcid.org/0000-0002-2964-2520
Naz R. Majeed Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq https://orcid.org/0000-0001-7354-5819
Aya A. Noori Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq https://orcid.org/0009-0007-7892-2637
Mina M. Mohammed Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq https://orcid.org/0009-0009-0909-3606
Washan N. Hamad Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq https://orcid.org/0000-0001-8121-6329
Adyan A. Mohammedsaeed Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq https://orcid.org/0009-0007-1427-3404

DOI: https://doi.org/10.24086/cuesj.v8n2y2024.pp56-64

Keywords: Klebsiella species, Antibiotic resistance, Biofilm formation, Extended-spectrum β-lactamase, Klebsiella pneumoniae

Abstract

The primary cause of nosocomial infections is Klebsiella pneumonia, an environmental gram-negative, encapsulated, non-motile bacterium. Antibiotic resistance and biofilm formation are two crucial characteristics that contribute to the pathogenicity of this bacterium. This research aimed to examine the relationship between antibiotic resistance and the capability of Klebsiella species isolated from Erbil hospital patients to produce biofilms. Therefore, in this study, 38 samples were collected from different laboratories and hospitals. At first, the samples were rejuvenated in Tryptic soy broth and then cultured on MacConkey agar. Gram staining and urease test were performed to identify the bacterial isolates while VITEK 2 system was performed to determine both the bacterial isolates and the antibiotic susceptibility. At last, Biofilm detection was assessed by the microliter plate technique as well as the Congo red agar method. The results revealed positive Klebsiella samples that were both resistant to some antibiotics and biofilm producers.

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Author Biographies

Ali M.Hussein, Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq

Ali M. Hussein is a Lecturer at the Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil. He has Ph.D. in Microbiology. His research interests are in Pathogenic Bacteria, Molecular Microbiology, and Biotechnology. Dr. Ali is also a member of the Microbiology Society journals.

Mohammed Issam Jameel, Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq

Mohammed Isam Jameel is a Assistant Lecturer at the Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil

Naz R. Majeed, Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq

Naz Rebaz Majeed is a college graduate. She has a B.Sc. degree from the Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq. Through her studies and researches at the Department of Biomedical Sciences, she has improved her productivity. Upon completion of her undergraduate studies, she intends to keep improving and mastering the vast array of knowledge that the biomedical sciences can provide.

Aya A. Noori, Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq

Aya Abdullah Noori is a college graduate from the Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq.

Mina M. Mohammed, Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq

Mina Mohsin Mohamad is a college graduate from the Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq.

Washan N. Hamad, Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq

Washan Nabaz Hamad is a Lab Assistant at the Department of Medical Laboratory Analysis, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq. She has a B.Sc. degree from the Department of Biomedical Sciences, College of Applied Science, Cihan University-Erbil, Kurdistan Region, Iraq.

Adyan A. Mohammedsaeed, Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq

Adyan Amir Mohammedsaeed is a college graduate from the Department of Biomedical Sciences, College of Applied Sciences, Cihan University-Erbil, Kurdistan Region, Iraq

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