Cytotoxic Effects of Pistacia khinjuk Seed Extracts on Different Cell Lines and its Mitogenic Effects on Blood Lymphocyte In Vitro
Reports indicated that extract Pistacia khinjuk has anti-inflammatory, antipyretic, antibacterial, and antiviral, in treating of diarrhea and throat infections and has hepatoprotective effects against acetaminophen and carbon tetrachloride. This study was undertaken to investigate the possible cytotoxic effects of methanolic and aqueous seeds extract of P. khinjuk on different tumors (rhabdomyosarcoma [RD] and murine mammary adenocarcinoma [Ahmed-Mohammed-Nahi-2003 (AMN-3)]) and normal cell lines (murine fibroblast) and its mitogenic effects on blood lymphocytes. The cytotoxic effects of P. khinjuk seed extracts were evaluated on two tumor cell lines, RD and murine mammary adenocarcinoma (AMN-3) and one normal cell line, murine fibroblast (L20B). Moreover, the mitogenic effects of the plant extract were studied, on human blood lymphocytes. Both methanolic and aqueous seed extracts of P. khinjuk significantly induced tumor cell lines and the normal cell line proliferation, especially in highest concentrations. The results show that the extracts induced significant increases in human blood lymphocyte proliferation at 72 h. This activity of plant extracts recommends it as a good mitogenic agent in researches; in conclusion, seed extracts of P. khinjuk induced proliferation of all tested cell lines. High concentrations of both aqueous and methanolic seed extracts of P. khinjuk showed mitogenic effects.
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