Green Synthesis of Gold Nanoparticles Using Zingiber Officinale Rhizomes Aqueous Extract and its Gastroprotective Activity Against Ethanol-Produced Stomach Ulcer in Rodents

  • Suhayla H. Shareef Department of Biology, College of Education, Salahaddin University, Erbil 44001, Kurdistan Region, Iraq, Department of Medical Microbiology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq https://orcid.org/0000-0002-7241-9885
  • Ismail N.M. Hadi Department of Medical Microbiology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq.
  • Mustafa A. Badeea ‎ Department of Medical Microbiology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq.
  • Mahmood A. Abdulla Department of Medical Microbiology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq. https://orcid.org/0000-0003-4784-6313
Keywords: Synthesized Zingiber officinale gold nanoparticle, gastric ulcer, histology, PAS

Abstract

Synthesis of gold nanoparticles exhibition wide range of bioactivities and was conventionally used for the remedy of numerous disorders. Our study aims to measure the gastroprotective effect of synthesized gold nanoparticles on absolute ethanol-induced stomach ulceration in rats. The rats were noticeably administered with two doses of gold nanoparticles (175 ppm and 350 ppm) with absolute alcohol and showed a significant improvement in the adverse effect of ethanol-produced stomach damage as confirmed by decreasing ulcer index and raising % of ulcer prevention. Increased mucus pH of stomach content reduced ulcerated portion, condensed or nonappearance of edema, and white blood cells permeation subcutaneous layer. In gastric tissue homogenate, synthesized gold nanoparticles presented a substantial increase in superoxide dismutase (SOD), catalase (CAT) actions, and a substantial lessening of malondialdehyde (MDA) amount. Besides, gold nanoparticle increases the intensity of periodic acid Schiff stained (PAS) of the gastric epithelium of the stomach. Gold nanoparticles confirmed gastro-protective effects which might be qualified to its antioxidant, increase mucus secretion, increase endogenous enzymes (SOD and CAT), and reduce MDA quantity.

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Author Biographies

Suhayla H. Shareef, Department of Biology, College of Education, Salahaddin University, Erbil 44001, Kurdistan Region, Iraq, Department of Medical Microbiology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq

Suhayla Hamad Shareef is a Lecturer at the Department of Medical Biochemical Analysis, College of Health Technology, Cihan University-Erbil. She got a B.Sc. degree in Biology Department/Salahaddin University-Erbil, the M.Sc. degree in College of Medicine / Hawler Medical University –Erbil, and a Ph.D. degree in College of Medicine/ University of Sulaimani. Her research interests are in Immunology, Histopathology, and Immunohistochemistry.

Ismail N.M. Hadi, Department of Medical Microbiology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq.

Ismail N. Mahdi just grauated with a B.Sc. at the Department of Medical Microbiology, College of Science, Cihan University-Erbil

Mustafa A. Badeea ‎, Department of Medical Microbiology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq.

Mustafa A. Badeea just graduated with  a B.Sc. at the Department of Medical Microbiology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq.

Mahmood A. Abdulla, Department of Medical Microbiology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq.

Mahmood Ameen Abdulla is a Professor at the Department of Medical Microbiology, College of Science, Cihan University-Erbil. He got a B.Sc. in Veterinary Medicine and Surgery University of Mosul in 1980, M.Sc.  Medical Immunology University of Mosul 1983, and Ph.D. in Veterinary Parasitology, University Putra Malaysia (UPM), Malaysia. His research interests are Histopathology, Immunology, and Immunohistochemistry.  

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Published
2022-08-22
How to Cite
1.
Shareef S, Hadi I, Badeea ‎M, Abdulla M. Green Synthesis of Gold Nanoparticles Using Zingiber Officinale Rhizomes Aqueous Extract and its Gastroprotective Activity Against Ethanol-Produced Stomach Ulcer in Rodents. cuesj [Internet]. 22Aug.2022 [cited 1Oct.2022];6(2):68-5. Available from: https://journals.cihanuniversity.edu.iq/index.php/cuesj/article/view/737
Section
Research Article