Effect of Isatis spp. Extraction on the Growth of Aspergillus niger and Candida albicans
Abstract
In this study, The crude extraction of Isatis spp. (I.tinctoria, I.busichaina and I. lusitanica) were investigated for their antifungal activity. Each of methanol, ethanol, and H2O was used as a solvent. Due to find the most effective part of each species, the plant parts were used separately. The aim of this research was to determine the effect of the natural product on two common pathogenic fungi Aspergillus niger & Candida albicans. The results show that most of the used plants have a significant effect on both used fungal species. Extracted each of flower, I. tinctoria was shown the best results comparing to the other used species. Extracted leaf and flower of I. tinctoria by methanol was show the best result on Aspergillus niger & Candida albicans. The growth zone was around 90mm for control and 61mm at 75% concentration of methanolic and ethanolic extraction. The flower was for followed by the stem. Depend on the results, the methanol was shown the highest number, then ethanol and the lowest inhibition zone area was for H2O extraction.
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References
Adiguzel , Ahmet; Sokmen Munevver; Ozkan Hakan and Agar Guleray.(2009). In vitro Antimicrobial and Antioxidant Activities of Methanol and Hexane Extract of Astragalus Species Growing in the Eastern Anatolia Region of Turkey.Turkish Journal of Biology ,33 , P: 65- 71.
Atlas, R.M.(1995).Laboratory Manual of Experimental Microbiology . Mosbyyear book, Inc., USA. P: 25.
Chen, M., Gan, L., Lin, S., Wang, X., Li, L., Li, Y., Li., Zhu, C., Wang, Y., Ji, B., Jiang, Ji., Yang, Y., Shi, J. (2012). Alkaloids from the root of Isatis indigotica. Journal of natural products, 75(6), 1167-1176.
Jethinlakhosh , J. P. and Lathika V. , (2012) . Screening of phytochemical constituents and antimicrobial activity of traditional medicinal plants. International Journal of Research in Pharmaceutical Sciences, 3(3) P: 461- 465.
Li, X., Feng, S., Farajtabar, A., Zhang, N., Chen, G., & Zhao, H. (2018). Solubility modelling, solvent effect and preferential solvation of 6-chloropurine in several aqueous co-solvent mixtures between 283.15 K and 328.15 K. The Journal of Chemical Thermodynamics, 127, 106-116.
Nivoix, Y., Launoy, A., Lutun, P., Moulin, J. C., Phai Pang, K. A., Fornecker, L. M., Ubeaud-Sequier, G. (2012). Adherence to recommendations for the use of antifungal agents in a tertiary care hospital. Journal of antimicrobial chemotherapy, 67(10), 2506-2513.
Pérez, C. C., García, P. G., Parra, L. G. B., Sánchez, S. P., & Méndez, J. D. (2019). Candida Lusitaniae. ISOLATION, IDENTIFICATION AND CLINICAL RELEVANCE.
Rispail, N., Philip, M.and Webb, K.J. (2005) Phenolic compound extraction and analysis. Lotus Japonicas hand book , P: 349-355.
Tane, P., S.D. Tatsimo, G.A. Ayimele and J.D. Connolly. 2005. Bioactive metabolites from Aframomum species. In: 11th NAPRECA Symposium Book of Proceedings, 214-223.
Van Wyk, C., Botha, F. S., Vleggaar, R., & Eloff, J. N. (2018). Obliquumol, a novel antifungal and a potential scaffold lead compound, isolated from the leaves of Ptaeroxylon obliquum (sneezewood) for treatment of Candida albicans infections. Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie, 37(1), 1-5.
Wang, H., Gao, G., Ke, L., Zhou, J., & Rao, P. (2018). Isolation and Characterization of a Lectin-like Protein (SBLP) from the Dried Roots of Scutellaria baicalensis (Lamiaceae). Natural Product Communications, 13(12), 1934578X1801301232.
Zhang, D., Li, J., Ruan, D., Chen, Z., Zhu, W., Shi, Y., Chen, K., Li, Y.,Wang, R. (2019). Lignans from Isatis indigotica roots and their inhibitory effects on nitric oxide production. Fitoterapia, 104189.
Zwickey, H., & Lipski, L. (2018). Expanding Our View of Herbal Medicine. The Journal of Alternative and Complementary Medicine, 24(7), 619-620.
Copyright (c) 2020 Ali M.Hussein, Zehra Odabaş-Serin, Zhala B. Taha
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