Immunohistochemical and Molecular Studies of p53 and KRAS Protein and Their Relations to Colorectal Carcinoma

  • Merza H. Homady Department of Biomedical Sciences, College of Science, Cihan University-Erbil, Kurdistan Region-Iraq https://orcid.org/0000-0002-8449-0865
  • Tanya S. Salih Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq
  • Mariamm M. Al-Jubori College of Medicine, Babylon University, Iraq
  • Mustafa D. Younus Department of General Biology, Cihan University-Erbil, Kurdistan Region, Iraq
Keywords: Kirsten rat sarcoma virus, carcinoma, stage, colorectal, histology

Abstract

The study inc1uded 50 tissue blocks embedded in paraffin wax (16 females and 34 males), obtained from a patients group with (CRC) colorectal cancer , as well as 35 Tissue blocks that were embedded in paraffin wax from norma1 co1on (ulcerative co1itis) as controls. A relatively few oncogenes and most prominently tumor-suppressing genes, Kirastien rat sarcoma virus (KRAS), and P53 genes have been mutated into a significant part of CRCs, and a broad collection of mutated genes has been defined in CRC subsets. Current findings showed very significant differences between patients and control subjects in the p53 positive rate (P<0.001). TP53 Pro/Pro genotype positivity was higher in the contro1 group I than in the patient group I and this was a significant difference (Pi<0.001) with an odd ratio of less than one. The genotype Pro/Pro was considered to be protective against colorectal carcinoma preventively fractured 0.767. The positive rate of p53 Arg/Arg genotype in patients was more frequent and statistically significant (P <0.01), because the odd ratio was more than one. The genotype Arg/Arg would be considered a colorectal carcinoma risk factor. We conclude that p53 over expression is used as an indicator of p53 mutation (as identified by immuno-historic chemistry) and KRAS protein expression was negatively impaired for all the patients in the current study.

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Published
2021-06-20
How to Cite
1.
Homady M, Salih T, Al-Jubori M, Younus M. Immunohistochemical and Molecular Studies of p53 and KRAS Protein and Their Relations to Colorectal Carcinoma. cuesj [Internet]. 20Jun.2021 [cited 20Oct.2021];5(1):28-3. Available from: https://journals.cihanuniversity.edu.iq/index.php/cuesj/article/view/385
Section
Research Article